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BL21 competent cells

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BL21 competent cells
Kompetente Zellen
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

  • High-level protein expression and easy induction
  • Can be used with non-T7 RNA polymerase protein expression systems
  • Allows the tightest control of protein expression for extremely toxic proteins
  • Suitable for expressing proteins from all species, including mammals
  • Ideal for difficult protein expression, especially when codon bias is a problem
  • Increases transformation efficiency with Hte phenotype
  • Derived from Agilent’s high performance BL21-Gold competent cell line

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins.

Agilent BL21-CodonPlus competent cells dramatically improve protein expression in E. coli by overcoming codon bias. Codon bias occurs when forced high-level expression of a gene containing codons rarely expressed in E. coli depletes internal tRNA pools. This often results in poor protein synthesis, early termination of the polypeptide cha