Immobilised Pepsin
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A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
- Generate F(ab)₂ fragments
- Eliminate contaminating pepsin enzyme
- Can be used in virtually all scenarios using free pepsin
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