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Anti-Biotin Mouse Monoclonal Antibody [clone: SPM375]

Lieferant: ProSci Inc.
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34-197
PRSI34-197EA 773 EUR
PRSI34-197
Anti-Biotin Mouse Monoclonal Antibody [clone: SPM375]
Antikörper
It recognises both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Confocal Microscopy Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 0,5 - 1 µg/ml In situ Nucleic Acid Hybridization Western: blot 1-3 µg/ml Immunohistochemistry (FFPE): 0,5 - 1 µg/ml for 30 minutes at RT (1) The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.


Type: Primary
Antigen: Biotin Antibody [SPM375]
Clonality: Monoclonal
Clone: SPM375
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: All species
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