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Artikel-Nr:
(233-5093)
Lieferant:
SLG KUNSTOFFABRIK UND FORMENBA
Hersteller-Artikelnummer::
394235
Lokale Artikelnummer::
SLGK394235
Beschreibung:
Besonders hochwertiges und langlebiges Hartkeramikmaterial für dauerhafte, besondere Schärfe. Ohne Metallteile, somit u.a. auch ideal geeignet für die Spurenanalytik. Superleichter, stabiler, ergonomisch geformter Griff für sicheres und einfaches Handling.
VE:
1 * 1 ST
Lieferant:
MP Biomedicals
Beschreibung:
Soluble in water (50 mg/ml - clear solution).
Lieferant:
COMBI-BLOCKS
Beschreibung:
2-Brom-5-fluorbenzamid
Artikel-Nr:
(SIAL147214-25G)
Lieferant:
Sigma-Aldrich
Hersteller-Artikelnummer::
147214-25G
Lokale Artikelnummer::
SIAL147214-25G
Beschreibung:
4-Amino-2-nitrophenol, Sigma-Aldrich®
VE:
1 * 25 g
Artikel-Nr:
(BOSSBS-1135R-A488)
Lieferant:
Bioss
Hersteller-Artikelnummer::
BS-1135R-A488
Lokale Artikelnummer::
BOSSBS-1135R-A488
Beschreibung:
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
VE:
1 * 100 µl
Artikel-Nr:
(BOSSBS-1135R-A750)
Lieferant:
Bioss
Hersteller-Artikelnummer::
BS-1135R-A750
Lokale Artikelnummer::
BOSSBS-1135R-A750
Beschreibung:
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
VE:
1 * 100 µl
Artikel-Nr:
(BOSSBS-1135R-A647)
Lieferant:
Bioss
Hersteller-Artikelnummer::
BS-1135R-A647
Lokale Artikelnummer::
BOSSBS-1135R-A647
Beschreibung:
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
VE:
1 * 100 µl
Artikel-Nr:
(BOSSBS-1135R-CY5.5)
Lieferant:
Bioss
Hersteller-Artikelnummer::
BS-1135R-CY5.5
Lokale Artikelnummer::
BOSSBS-1135R-CY5.5
Beschreibung:
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
VE:
1 * 100 µl
Lieferant:
Sigma-Aldrich
Beschreibung:
Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) is a water soluble, cytocompatible, Type I photoinitiator for use in the polymerization of hydrogels or other polymeric materials.
Artikel-Nr:
(201-8628)
Lieferant:
SciLabware
Hersteller-Artikelnummer::
MF24/5
Lokale Artikelnummer::
BIBBMF24/5
Beschreibung:
Pyrex® borosilicate glass.
VE:
1 * 5 ST
Artikel-Nr:
(PROOTRC-G855000-5G)
Lieferant:
LGC Standards PROMOCHEM
Hersteller-Artikelnummer::
TRC-G855000-5G
Lokale Artikelnummer::
PROOTRC-G855000-5G
Beschreibung:
L-(+)-Gulonic acid-γ-lactone
VE:
1 * 5 g
Lieferant:
Sigma-Aldrich
Beschreibung:
L(+)-Ascorbinsäure, Sigma-Aldrich®
Artikel-Nr:
(ENAMEN300101066.1G)
Lieferant:
SIA ENAMINE
Hersteller-Artikelnummer::
EN300101066.1G
Lokale Artikelnummer::
ENAMEN300101066.1G
Beschreibung:
3-(2-Carbamoylphenoxy)propionsäure
VE:
1 * 1 g
 This is a MarketSource item. Additional charges may apply
Artikel-Nr:
(J61745.MF)
Lieferant:
Alfa Aesar
Hersteller-Artikelnummer::
J61745.MF
Lokale Artikelnummer::
ALFAJ61745.MF
Beschreibung:
(-)-Epigallocatechin gallate
VE:
1 * 50 mg
Artikel-Nr:
(SIAL239216-100G)
Lieferant:
Sigma-Aldrich
Hersteller-Artikelnummer::
239216-100G
Lokale Artikelnummer::
SIAL239216-100G
Beschreibung:
Calciumcarbonat ≥99% ACS
VE:
1 * 100 g
Preis auf Anfrage
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 noch angezeigt wird und Sie Hilfe benötigen, rufen Sie uns an 1-800-932 - 5000.
Lager für diesen Artikel ist begrenzt, kann aber in einem Lagerhaus in Ihrer Nähe zur Verfügung. Bitte stellen Sie sicher, dass Sie in sind angemeldet auf dieser Seite, so dass verfügbare Bestand angezeigt werden können. Wenn das
noch angezeigt wird und Sie Hilfe benötigen, rufen Sie uns an 1-800-932 - 5000.
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