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Ethidiumbromid 0,625 mg/l in wässriger Lösung, Biotechnologische Qualität

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E406-5MLEA 36.3 EUR InStock
E406-5ML
Ethidiumbromid 0,625 mg/l in wässriger Lösung, Biotechnologische Qualität
Ethidiumbromid
Ethidiumbromid in 0,625 mg/ml Tropfflasche. Ein Tropfen in 50 ml flüssiger Agarose oder Puffer verdünnt ergibt eine Endkonzentration von 0,5 μg/ml. In einer Konzentration von 0,5 μg/ml ist Ethidiumbromid zweckmäßig in Agarose- oder Acrylamidgelen für die Bandenfeststellung. Es ermöglicht die Detektion von Nukleinsäure in Mengen von lediglich 10 ng. In höheren Konzentrationen (0,5 - 1,0 μg/ml) kann es bei der Aufreinigung von DNA in einem Caesiumchlorid-Gradienten verwendet werden.

  • Used to detect as little as 10 ng of nucleic acid
  • Dropper bottles are shatter-resistant and light-proof
  • No additional hazardous shipping costs are applied to ethidium bromide dropper bottles

At a concentration of 0,5 μg/ml, ethidium bromide is useful in agarose or acrylamide gels for band detection.

At higher concentrations (0,5 to 1,0 μg/ml), it may be used to facilitate purification of DNA in a caesium chloride gradient.

One drop diluted into 50 ml of molten agarose or buffer yields a final concentration of 0,5 μg/ml.

Verpackung: In 0,625 mg/ml dropper bottles.


Formel: C₂₁H₂₀BrN₃
MW: 394.31 g/mol
Siedepunkt: 72
 MDL Number: MFCD00011724
CAS-Nummer: 1239-45-8
EINECS: 214-984-6
Merck Index: 13,04751
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Spezifikation Testergebnisse
Abs.@ Lambda Max 0,68 - 0,74
DNase none detected
Lambda Max (8ml/100ml Wasser) 478 - 482 nm
RNase none detected

 

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The customer is responsible for unloading and transporting large and/or heavy items from delivery vans and for supervising the unloading of all other products delivered.

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