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Protein Extraction Kits
Artikel-Nr:
(786-260)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-260
Lokale Artikelnummer::
GENO786-260
Beschreibung:
FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
VE:
1 * 1 KIT
Artikel-Nr:
(786-250)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-250
Lokale Artikelnummer::
GENO786-250
Beschreibung:
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
VE:
1 * 1 KIT
Artikel-Nr:
(786-258)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-258
Lokale Artikelnummer::
GENO786-258
Beschreibung:
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
VE:
1 * 1 KIT
Lieferant:
G-Biosciences
Beschreibung:
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
Artikel-Nr:
(786-022)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-022
Lokale Artikelnummer::
GENO786-022
Beschreibung:
FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
VE:
1 * 1 KIT
Artikel-Nr:
(786-360)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-360
Lokale Artikelnummer::
GENO786-360
Beschreibung:
Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.
VE:
1 * 1 KIT
Lieferant:
Thermo Scientific
Beschreibung:
Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.
Lieferant:
Biotium
Beschreibung:
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).
Artikel-Nr:
(786-259)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-259
Lokale Artikelnummer::
GENO786-259
Beschreibung:
Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
VE:
1 * 1 KIT
Artikel-Nr:
(786-247)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-247
Lokale Artikelnummer::
GENO786-247
Beschreibung:
FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
VE:
1 * 1 KIT
Artikel-Nr:
(786-253)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-253
Lokale Artikelnummer::
GENO786-253
Beschreibung:
FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
VE:
1 * 1 KIT
Artikel-Nr:
(786-018)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-018
Lokale Artikelnummer::
GENO786-018
Beschreibung:
FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
VE:
1 * 1 KIT
Artikel-Nr:
(786-246)
Lieferant:
G-Biosciences
Hersteller-Artikelnummer::
786-246
Lokale Artikelnummer::
GENO786-246
Beschreibung:
Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
VE:
1 * 1 KIT
Artikel-Nr:
(PIER88279)
Lieferant:
Thermo Scientific
Hersteller-Artikelnummer::
88279
Lokale Artikelnummer::
PIER88279
Beschreibung:
The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
VE:
1 * 1 KIT
Lieferant:
Thermo Scientific
Beschreibung:
B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).
Lieferant:
G-Biosciences
Beschreibung:
Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
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 noch angezeigt wird und Sie Hilfe benötigen, rufen Sie uns an 1-800-932 - 5000.
Lager für diesen Artikel ist begrenzt, kann aber in einem Lagerhaus in Ihrer Nähe zur Verfügung. Bitte stellen Sie sicher, dass Sie in sind angemeldet auf dieser Seite, so dass verfügbare Bestand angezeigt werden können. Wenn das
noch angezeigt wird und Sie Hilfe benötigen, rufen Sie uns an 1-800-932 - 5000.
Dieses Produkt kann nur an eine Lieferadresse versandt werden die über die entsprechende Lizenzen verfügt. Für weitere Hilfe bitte kontaktieren Sie Ihr VWR Vertriebszentrum.
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