50044
50043
50043-1
50044.EA
293
EUR
InStock
50044.
50043.
50043-1
Indo-1 AM ≥96% (durch HPLC) fluoreszierender Indikator, zur Kalziumbestimmung
Indo-1 AM
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1-AM Ester itself does not bind Ca2+ , but it is readily hydrolyzed to indo-1 by endogenous esterases once it enters cells.
- Absorption/Emission 356 nm/478 nm (no Ca2+); 330 nm/400 nm (high Ca2+)
- Extinction Coefficient 33,000 M -1 cm -1 (no Ca2+); 33,000 M -1 cm -1 (high Ca2+)
- Cell permeability: Membrane permeant
- Light yellow solid soluble in DMSO
- Store desiccated at -20°C and protect from light, especially in solution