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Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®

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M-Clarity™ Qualitätsstufe = MQ200

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T3163-10MGEA 790 EUR InStock
T3163-10MG T3163-5MG
Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®
Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R)
Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

  • TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry
  • Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands
  • TRITC-antibodies are used to identify pathogenic amoebae
  • TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms
  • TRITC-Iabeled reagents have also been used as fluorescent probes of live cells
  • Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior


Formel: C₂₅H₂₁N₃O₃S
Storage Temperature: Kühlschrank
 MDL Number: MFCD00063517
CAS-Nummer: 80724-20-5
https://qa.digitalassets.avantorsciences.com/adaptivemedia/rendition?id=97a45e9478d0fb20397121171b222931da085d03&vid=97a45e9478d0fb20397121171b222931da085d03&prid=web&clid=SAPDAM
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Spezifikation Testergebnisse
Appearance (Form) Powder
Solubility (Colour) Faint Red to Very Dark Red to Very Dark Purple
Solubility (Form)
10 mg/mL , 1M NH4OH in MeOH		 Solution
1H NMR Spectrum Conforms to Structure
Solvent Content (by GC) ≤4 %
Nitrogen ≥ 7.5 %
Dye Content ≥80 %
Labeling Efficiency ≥50 %

 

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