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Gibson Assembly® CLONING

DNA MY WAY™ Get DNA your way. Generate seamless plasmid DNA or BAC clones without restriction digestion or DNA scars.

Gibson Assembly® cloning, developed by the J. Craig Venter Institute and Synthetic Genomics, allows for insertion of DNA fragments into virtually any vector without the need for compatible restriction sites. Join any two fragments to generate seamless constructs in your desired vector.

  • Significantly faster than traditional cloning methods
  • Avoid time-consuming subcloning
  • Insert 1-15 fragments in a single round of cloning in ~1 hour
  • Achieve transformation efficiencies of up to 95%
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Download Cloning Guide