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Improving DNA and RNA co-extraction from Formalin-fixed paraffin-embedded (FFPE) samples

Webinar

Tuesday, 15th September, 2020
15:00 to 16:00 CEST (Berlin, Paris, Madrid)

Replay

Obtaining high quality and high yielding DNA or RNA is challenging since nucleic acids in FFPE tissue samples are often fragmented and prone to chemical modifications. Co-extraction is beneficial as samples may be limited. While there are many kits to purify either the DNA or RNA from FFPE samples, only a few can purify both DNA and RNA from the same sample.

In this webinar, we look at comparisons of three nucleic acid isolation kits and learn about the different nucleic acid extraction methodology to co-extract DNA and RNA from the same FFPE sample and comparing analysis of their yield, quality, and NGS data.

Presented by:

Nelson Cotrim

Nelson Cotrim (MSc) is a Product and Application Scientist at Omega Bio-tek, Inc, responsible for providing technical support for customers and distributors as well as installing, validating and training of nucleic acid purification solutions on high throughput automated platforms. Previously, he served as Research Assistant at Oxford Nanoimaging and Development Scientist at Healthcare Diagnostics where he developed the point of care diagnostic devices based on either nucleic acid amplification or using single-molecule fluorescence techniques. Nelson obtained his Masters of Science in Genetics and Molecular Biology from the University of São Paulo, Brazil working with human genetic diseases and population genetics in the African-Brazilian isolated population. He has served as a Genetic Technologist at the Institute of Medical Genetics analyzing results of NGS and Sanger sequencing assays for human genetic disease diagnostics. He has also authored several peer-reviewed journal papers.